Immunobiology CRISPR Core

Mission Statement

The mission of the CRISPR core is to provide Yale University research groups and the academic research community at large access to the latest technologies available for introducing targeting genome alterations for the production of novel mouse models.

Background

The CRISPR/Cas9 technology is a new method that has revolutionized the way genome editing is performed. This technology is based on the capability of the S. Pyogenes Cas9 endonuclease to cleave specific DNA sequences guided by small RNAs (gRNAs) and donor DNAs that promote homologous directed repair. The Cas9 technology allows you to perform all the possible targeting events that are usually achieved (i.e knockouts and conditional knockouts) through traditional methods in a faster and more economical manner.

Services and fee structure per attempt

CRISPR/Cas9: Simple knockout and Conditional knockout editing are per attempt*. PI’s are required to provide a PTAEO and an IACUC protocol number at the time a project is initiated to cover all daily census fees post weaning.

 gRNA design construction, LoxP or Tag design 
Internal Yale$5,952.00
External Yale$10,058.00
Construct design 
Internal Yale$575.00
External Yale$972.00
Genotyping surveyor 
Internal Yale$575.00
External Yale$972.00

Should Surveyor mismatch analysis be required to evaluate guide function, an additional $200 will be assessed for Yale members and $322.00 for external per analysis. 

* All payments are required in advance and are per attempt. Success is not guaranteed: the success of a project depends in part on the efficiency of any particular guide selection, which can be predicted only in theory and requires empirical determination. Statistical failure to achieve the correct genotype requires guide redesign and re-injection, all of which takes time. But the most important consideration is the complexity of the project. Simple deletions are relatively straightforward, as are point mutations. Multiple changes and large insertions are lower efficiency and multiple attempts. 

By submitting this form you are authorizing on behalf of the PI the amount designed to be charged to the award provided.