Mission StatementThe mission of the CRISPR core is to provide Yale University research groups and the academic research community at large access to the latest technologies available for introducing targeting genome alterations for the production of novel mouse models.
BackgroundThe CRISPR/Cas9 technology is a new method that has revolutionized the way genome editing is performed. This technology is based on the capability of the S. Pyogenes Cas9 endonuclease to cleave specific DNA sequences guided by small RNAs (gRNAs) and donor DNAs that promote homologous directed repair. The Cas9 technology allows you to perform all the possible targeting events that are usually achieved (i.e knockouts and conditional knockouts) through traditional methods in a faster and more economical manner.
Services and fee structure per attempt
CRISPR/Cas9: Simple knockout and Conditional knockout editing are per attempt*. PI’s are required to provide a PTAEO and an IACUC protocol number at the time a project is initiated to cover all daily census fees post weaning.
|gRNA design construction, LoxP or Tag design|
By submitting this form you are authorizing on behalf of the PI the amount designed to be charged to the award provided.